ID

45752

Descrizione

Principal Investigator: Theodore J. Price, School of Behavioral and Brain Sciences, The University of Texas at Dallas, Richardson, TX, USA MeSH: Chronic Pain,Neuralgia https://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/study.cgi?study_id=phs001158 RNA sequencing was performed on human DRGs and relative gene abundances were calculated. Various analyses were performed: - Human DRG gene expression profiles were contrasted with a panel of gene expression profiles of relevant tissues in human and mouse ( integrating, among other sources, datasets from ENCODE and GTex ) in order to identify. ol type="a"- DRG-enriched gene expression, co-expression modules of DRG-expressed genes, and key transcriptional regulators in humans. - Contrasting the human and mouse DRG transcriptomes to identify DRG-enriched gene expression patterns that were conserved between human and mouse, identifying putative cell types of expression of these genes, and potential known drugs that might target the corresponding gene products. - Characterization of non-coding RNA profile of human and mouse DRGs. - Characterization of DRG-enriched alternative splicing and alternative transcription start site usage based transcript variants in humans and mouse, and the overlap between these two species. - Contrasting of human DRG and GTex human tibial nerve samples to identify putative axonally transported mRNAs in sensory neurons. - Human DRG transcriptomes from donors suffering from neuropathic and/or chronic pain were contrasted with controls to identify. ol type="a"- Differentially expressed genes, pathways and regulators path play a potential role in neuronal plasticity, electrophysiological activity, immune signaling and response. liPredictive models (Random Forests) were built to jointly predict the sex and pain state of samples based on information contained solely in autosomal gene expression profile./li liGene co-expression modules were identified and gene set enrichment analysis performed.to identify sample - pathway associations, and to broadly characterize plasticity in human DRG cell types./li /ol

collegamento

dbGaP-study=phs001158

Keywords

versioni (1)

1. 05/06/23 05/06/23 - Chiara Middel

Titolare del copyright

Theodore J. Price, School of Behavioral and Brain Sciences, The University of Texas at Dallas, Richardson, TX, USA

Caricato su

5 giugno 2023

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