ID
45752
Description
Principal Investigator: Theodore J. Price, School of Behavioral and Brain Sciences, The University of Texas at Dallas, Richardson, TX, USA MeSH: Chronic Pain,Neuralgia https://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/study.cgi?study_id=phs001158 RNA sequencing was performed on human DRGs and relative gene abundances were calculated. Various analyses were performed: - Human DRG gene expression profiles were contrasted with a panel of gene expression profiles of relevant tissues in human and mouse ( integrating, among other sources, datasets from ENCODE and GTex ) in order to identify. ol type="a"- DRG-enriched gene expression, co-expression modules of DRG-expressed genes, and key transcriptional regulators in humans. - Contrasting the human and mouse DRG transcriptomes to identify DRG-enriched gene expression patterns that were conserved between human and mouse, identifying putative cell types of expression of these genes, and potential known drugs that might target the corresponding gene products. - Characterization of non-coding RNA profile of human and mouse DRGs. - Characterization of DRG-enriched alternative splicing and alternative transcription start site usage based transcript variants in humans and mouse, and the overlap between these two species. - Contrasting of human DRG and GTex human tibial nerve samples to identify putative axonally transported mRNAs in sensory neurons. - Human DRG transcriptomes from donors suffering from neuropathic and/or chronic pain were contrasted with controls to identify. ol type="a"- Differentially expressed genes, pathways and regulators path play a potential role in neuronal plasticity, electrophysiological activity, immune signaling and response. liPredictive models (Random Forests) were built to jointly predict the sex and pain state of samples based on information contained solely in autosomal gene expression profile./li liGene co-expression modules were identified and gene set enrichment analysis performed.to identify sample - pathway associations, and to broadly characterize plasticity in human DRG cell types./li /ol
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Versions (1)
- 05/06/2023 05/06/2023 - Chiara Middel
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Theodore J. Price, School of Behavioral and Brain Sciences, The University of Texas at Dallas, Richardson, TX, USA
Téléchargé le
5 juin 2023
DOI
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Creative Commons BY 4.0
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dbGaP phs001158 Human Dorsal Root Ganglion RNA Seq Profiling
Subject ID, age, race, presence and details of axial spine pain, presence and details of neuropathy / radiculopathy, type of tissue donation procedure, presence and duration of pain, presence and details of length dependent peripheral neuropathy, presence and details of nerve root compression, presence / absence of neuropathy in dermatome associated with DRG, presence and details of spinal cord compression, visual analogue scale values at maximum intensity (numeric/string), details of radiation therapy, details of chemotherapy or other systemic treatments, details of recent opioid or neuromodulatory drug use, and DRG extraction surgery details of participants with or without neuropathic and chronic pain and involved in the "Human Dorsal Root Ganglion RNA Landscape Profiling for Neuropathic and Chronic Pain" project.
- StudyEvent: SEV1
- Eligibility Criteria
- Subject ID, consent group, subject source, source subject ID, and affection status of participants with or without neuropathic and chronic pain and involved in the "Human Dorsal Root Ganglion RNA Landscape Profiling for Neuropathic and Chronic Pain" project.
- Subject ID, sample ID, sample source, source sample ID, and sample use variable obtained from participants with or without neuropathic and chronic pain and involved in the "Human Dorsal Root Ganglion RNA Landscape Profiling for Neuropathic and Chronic Pain" project.
- Subject ID, age, race, presence and details of axial spine pain, presence and details of neuropathy / radiculopathy, type of tissue donation procedure, presence and duration of pain, presence and details of length dependent peripheral neuropathy, presence and details of nerve root compression, presence / absence of neuropathy in dermatome associated with DRG, presence and details of spinal cord compression, visual analogue scale values at maximum intensity (numeric/string), details of radiation therapy, details of chemotherapy or other systemic treatments, details of recent opioid or neuromodulatory drug use, and DRG extraction surgery details of participants with or without neuropathic and chronic pain and involved in the "Human Dorsal Root Ganglion RNA Landscape Profiling for Neuropathic and Chronic Pain" project.
- Sample ID, analyte type, body site where sample was obtained, tumor status of sample, details of sample assay library type, sequencing center, sequencing platform, genotyping center, and spinal disk(s) and side on which sample was attached (L = Lumbar,T=Thoracic) of participants with or without neuropathic and chronic pain and involved in the "Human Dorsal Root Ganglion RNA Landscape Profiling for Neuropathic and Chronic Pain" project.
Similar models
Subject ID, age, race, presence and details of axial spine pain, presence and details of neuropathy / radiculopathy, type of tissue donation procedure, presence and duration of pain, presence and details of length dependent peripheral neuropathy, presence and details of nerve root compression, presence / absence of neuropathy in dermatome associated with DRG, presence and details of spinal cord compression, visual analogue scale values at maximum intensity (numeric/string), details of radiation therapy, details of chemotherapy or other systemic treatments, details of recent opioid or neuromodulatory drug use, and DRG extraction surgery details of participants with or without neuropathic and chronic pain and involved in the "Human Dorsal Root Ganglion RNA Landscape Profiling for Neuropathic and Chronic Pain" project.
- StudyEvent: SEV1
- Eligibility Criteria
- Subject ID, consent group, subject source, source subject ID, and affection status of participants with or without neuropathic and chronic pain and involved in the "Human Dorsal Root Ganglion RNA Landscape Profiling for Neuropathic and Chronic Pain" project.
- Subject ID, sample ID, sample source, source sample ID, and sample use variable obtained from participants with or without neuropathic and chronic pain and involved in the "Human Dorsal Root Ganglion RNA Landscape Profiling for Neuropathic and Chronic Pain" project.
- Subject ID, age, race, presence and details of axial spine pain, presence and details of neuropathy / radiculopathy, type of tissue donation procedure, presence and duration of pain, presence and details of length dependent peripheral neuropathy, presence and details of nerve root compression, presence / absence of neuropathy in dermatome associated with DRG, presence and details of spinal cord compression, visual analogue scale values at maximum intensity (numeric/string), details of radiation therapy, details of chemotherapy or other systemic treatments, details of recent opioid or neuromodulatory drug use, and DRG extraction surgery details of participants with or without neuropathic and chronic pain and involved in the "Human Dorsal Root Ganglion RNA Landscape Profiling for Neuropathic and Chronic Pain" project.
- Sample ID, analyte type, body site where sample was obtained, tumor status of sample, details of sample assay library type, sequencing center, sequencing platform, genotyping center, and spinal disk(s) and side on which sample was attached (L = Lumbar,T=Thoracic) of participants with or without neuropathic and chronic pain and involved in the "Human Dorsal Root Ganglion RNA Landscape Profiling for Neuropathic and Chronic Pain" project.
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