ID
45865
Description
Principal Investigator: Boaz P. Levi, PhD, Allen Institute for Brain Science, Seattle, WA, USA MeSH: Neuroglia,Growth and Development https://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/study.cgi?study_id=phs001016 The human neocortex is created from diverse intermixed progenitors in the prenatal germinal zones. These progenitors have been difficult to characterize since progenitors - particularly radial glia (RG) - are rare, and are defined by a combination of intracellular markers, position and morphology. To circumvent these problems we developed a method called FRISCR (Fixed and Recovered Intact Single Cell RNA) for transcriptome profiling of individual fixed, stained and sorted cells. We developed and validated FRISCR on human embryonic stem cells. We then profiled primary human RG (96 - 132 days post conception) that constitute only 1% of the mid-gestation cortex. These RG could be classified into ventricular zone-enriched RG (vRG) that express ANXA1 and CRYAB, and outer subventricular zone-localized RG (oRG) that express HOPX. Our study identifies the first markers and molecular profiles of vRG and oRG cells, and provides an essential step for understanding molecular networks driving the lineage of human neocortical progenitors. *Reprinted from Thomsen et. al. Nature Methods (2015), with permission from Nature Publishing.* Human embryonic stem cell data may be obtained through NCBI's GEO database, using accession number GSE71858. Raw data from one human sample that was not consented to be released to dbGaP may be obtained directly from the authors of Thomsen et. al., 2015.
Link
Keywords
Versions (1)
- 10/14/23 10/14/23 - Simon Heim
Copyright Holder
Boaz P. Levi, PhD, Allen Institute for Brain Science, Seattle, WA, USA
Uploaded on
October 14, 2023
DOI
To request one please log in.
License
Creative Commons BY 4.0
Model comments :
You can comment on the data model here. Via the speech bubbles at the itemgroups and items you can add comments to those specificially.
Itemgroup comments for :
Item comments for :
In order to download data models you must be logged in. Please log in or register for free.
dbGaP phs001016 Fixed Single Cell Study (Radial Glia Cells)
Eligibility Criteria
- StudyEvent: SEV1
- Eligibility Criteria
- Subject - Consent Information
- Subject - Sample Mapping - Sample Use Information
- The dataset includes sociodemographic (i.e. estimated fetal age in days/sex/post-mortem interval in hours) data not shown on the public web pages (because there are fewer than n=5 subjects), but available to authorized access users of the data.
- Sample - Attribute Information
Similar models
Eligibility Criteria
- StudyEvent: SEV1
- Eligibility Criteria
- Subject - Consent Information
- Subject - Sample Mapping - Sample Use Information
- The dataset includes sociodemographic (i.e. estimated fetal age in days/sex/post-mortem interval in hours) data not shown on the public web pages (because there are fewer than n=5 subjects), but available to authorized access users of the data.
- Sample - Attribute Information
C0680251 (UMLS CUI [1,2])
C2003903 (UMLS CUI [1,2])
C0269439 (UMLS CUI [1,3])
C0015965 (UMLS CUI [1,4])
C4035395 (UMLS CUI [1,5])
C0497552 (UMLS CUI [1,6])