0 Bedömningar

ID

45802

Beskrivning

Principal Investigator: Richard K. Wilson, PhD, McDonnell Genome Institute, Washington University School of Medicine, St. Louis, MO, USA MeSH: Neoplasms,Leukemia https://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/study.cgi?study_id=phs001066 We used massively parallel sequencing technology to profile the genomic DNA and RNA of tumor cells (leukemic bone marrow) and normal cells (skin biopsy) obtained from a single patient with Acute Lymphoblastic Leukemia (ALL), referred to throughout this study as 'ALL1'. Included in this study are samples obtained from a primary tumor, first relapse, second relapse and several intermediate timepoints. We identified somatic mutations present in each tumor by analysis of whole genome (WGS) and exome sequence data. Single nucleotide variants (SNVs) and small insertions and deletions were identified in both the exome and WGS data. Large copy number variations (CNVs) and structural variants (SVs) were identified in the WGS data. A custom capture reagent was designed to target most variants and used to generate deep validation sequence data. The expression status of all somatic variants was assessed by RNA-seq. The RNA-seq data was also used for gene expression analysis and gene fusion detection.

Länk

dbGaP-study=phs001066

Nyckelord

  1. 2023-06-23 2023-06-23 - Chiara Middel
Rättsinnehavare

Richard K. Wilson, PhD, McDonnell Genome Institute, Washington University School of Medicine, St. Louis, MO, USA

Uppladdad den

23 juni 2023

DOI

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Licens

Creative Commons BY 4.0

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    dbGaP phs001066 DNA and RNA Sequence of an Acute Lymphoblastic Leukemia

    Subject ID and gender of participants with acute lymphoblastic leukemia and involved in the "Whole genome, exome, transcriptome and validation sequencing of an acute lymphoblastic leukemia" project.

    pht005282
    Beskrivning

    pht005282

    Alias
    UMLS CUI [1,1]
    C3846158 (Other Coding)
    LOINC
    LA4728-7
    De-identified subject ID
    Beskrivning

    SUBJID

    Datatyp

    string

    Alias
    UMLS CUI [1,1]
    C4684638 (De-identified Information)
    UMLS CUI [1,2]
    C2348585 (Clinical Trial Subject Unique Identifier)
    Sex of subject
    Beskrivning

    SEX

    Datatyp

    string

    Alias
    UMLS CUI [1,1]
    C0079399 (Gender)
    SNOMED
    263495000
    LOINC
    LP61312-2

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