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ID

45802

Description

Principal Investigator: Richard K. Wilson, PhD, McDonnell Genome Institute, Washington University School of Medicine, St. Louis, MO, USA MeSH: Neoplasms,Leukemia https://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/study.cgi?study_id=phs001066 We used massively parallel sequencing technology to profile the genomic DNA and RNA of tumor cells (leukemic bone marrow) and normal cells (skin biopsy) obtained from a single patient with Acute Lymphoblastic Leukemia (ALL), referred to throughout this study as 'ALL1'. Included in this study are samples obtained from a primary tumor, first relapse, second relapse and several intermediate timepoints. We identified somatic mutations present in each tumor by analysis of whole genome (WGS) and exome sequence data. Single nucleotide variants (SNVs) and small insertions and deletions were identified in both the exome and WGS data. Large copy number variations (CNVs) and structural variants (SVs) were identified in the WGS data. A custom capture reagent was designed to target most variants and used to generate deep validation sequence data. The expression status of all somatic variants was assessed by RNA-seq. The RNA-seq data was also used for gene expression analysis and gene fusion detection.

Lien

dbGaP-study=phs001066

Mots-clés

  1. 23/06/2023 23/06/2023 - Chiara Middel
Détendeur de droits

Richard K. Wilson, PhD, McDonnell Genome Institute, Washington University School of Medicine, St. Louis, MO, USA

Téléchargé le

23 juin 2023

DOI

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Licence

Creative Commons BY 4.0

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    dbGaP phs001066 DNA and RNA Sequence of an Acute Lymphoblastic Leukemia

    Subject ID, sample ID, and sample use variable obtained from participants with acute lymphoblastic leukemia and involved in the "Whole genome, exome, transcriptome and validation sequencing of an acute lymphoblastic leukemia" project.

    pht005281
    Description

    pht005281

    Alias
    UMLS CUI [1,1]
    C3846158 (Other Coding)
    LOINC
    LA4728-7
    De-identified subject ID
    Description

    SUBJID

    Type de données

    string

    Alias
    UMLS CUI [1,1]
    C4684638 (De-identified Information)
    UMLS CUI [1,2]
    C2348585 (Clinical Trial Subject Unique Identifier)
    Sample ID
    Description

    SAMPID

    Type de données

    string

    Alias
    UMLS CUI [1,1]
    C1299222 (Sample identification number)
    SNOMED
    372274003
    Sample Use
    Description

    SAMPLE_USE

    Type de données

    string

    Alias
    UMLS CUI [1,1]
    C2347026 (Biospecimen)
    UMLS CUI [1,2]
    C1524063 (Use of)
    SNOMED
    260676000

    Similar models

    Subject ID, sample ID, and sample use variable obtained from participants with acute lymphoblastic leukemia and involved in the "Whole genome, exome, transcriptome and validation sequencing of an acute lymphoblastic leukemia" project.

    Name
    Type
    Description | Question | Decode (Coded Value)
    Type de données
    Alias
    Item Group
    pht005281
    C3846158 (UMLS CUI [1,1])
    SUBJID
    Item
    De-identified subject ID
    string
    C4684638 (UMLS CUI [1,1])
    C2348585 (UMLS CUI [1,2])
    SAMPID
    Item
    Sample ID
    string
    C1299222 (UMLS CUI [1,1])
    Item
    Sample Use
    string
    C2347026 (UMLS CUI [1,1])
    C1524063 (UMLS CUI [1,2])
    Code List
    Sample Use
    CL Item
    Custom targeted DNA sequencing (Seq_DNA_Target)
    CL Item
    Whole exome sequencing (Seq_DNA_WholeExome)
    C3640077 (UMLS CUI [1,1])
    CL Item
    Whole genome sequencing (Seq_DNA_WholeGenome)
    C3640076 (UMLS CUI [1,1])
    CL Item
    Whole transcriptome sequencing (Seq_RNA)
    C4086963 (UMLS CUI [1,1])

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