ID
45651
Description
Principal Investigator: Jennie L. Close, PhD, Allen Institute for Brain Science, Seattle, WA, USA MeSH: Growth and Development https://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/study.cgi?study_id=phs001276 GABAergic interneurons are essential for neural circuit function and their loss or dysfunction is implicated in human neuropsychiatric disease. In vitro methods for interneuron generation hold promise for studying human cellular and functional properties and ultimately therapeutic cell replacement. We used a protocol for generating cortical interneurons from hESCs and analyzed the properties and maturation timecourse of cell types using single-cell RNAseq (data available at GEO under: GSE93802 on March 10 2017). Transcriptomic profiles of the hESC-derived interneurons were compared to several different populations of cells from mid-gestation human neocortex that showed differing levels of PAX6 and SOX2 expression. For this study, 104 samples of 100 human neocortical cells each, have been sorted based on SOX2 and PAX6 expression, mRNA recovered from the fixed cells by FRISCR, and transcriptomic profiles generated by SmartSeq2. The RNA-seq data from the 104 100-cell samples is included in this dbGaP study.
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- 3/20/23 3/20/23 - Simon Heim
Copyright Holder
Jennie L. Close, PhD, Allen Institute for Brain Science, Seattle, WA, USA
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March 20, 2023
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Creative Commons BY 4.0
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dbGaP phs001276 Single-cell profiling of hESC-derived interneuron development
Eligibility Criteria
- StudyEvent: SEV1
- Eligibility Criteria
- Subject - Consent - Information
- Subject - Sample Mapping - Sample Use information
- The dataset provides gestation (gestational) age, gender and post-mortem index information ofcell donors.
- Sample Attribute Information includes RNA expression data information for genes PAX6 and SOX2.
Similar models
Eligibility Criteria
- StudyEvent: SEV1
- Eligibility Criteria
- Subject - Consent - Information
- Subject - Sample Mapping - Sample Use information
- The dataset provides gestation (gestational) age, gender and post-mortem index information ofcell donors.
- Sample Attribute Information includes RNA expression data information for genes PAX6 and SOX2.
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