ID

45484

Beschreibung

Principal Investigator: Matthew Meyerson, MD, PhD, Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts, MA, USA MeSH: Intestinal Neoplasms https://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/study.cgi?study_id=phs000579 The diagnosed incidence of small bowel neuroendocrine tumors (NETs) is increasing. While patients with localized disease can be treated surgically, those with metastatic disease currently have few treatment options. The success of biologically targeted therapies in other malignancies has led to interest in the molecular alterations underlying the pathogenesis of these NETs. To identify genetic aberrations in small intestine NETs, we generated copy number profiles from 31 primary and metastatic tumors and performed whole-exome sequencing on a subset of 29 primary small intestine NETs and 24 metastatic NETs in parallel with normal blood DNA. Whole-genome sequencing data was generated on 15 tumor/normal pairs and 5 primary/metastasis/normal trios. The global genetic landscape of small bowel NETs is relatively quiet. Consistent with previous studies, the overwhelming majority of tumors were characterized by loss of chromosome 18 and, to a lesser extent, other chromosome arm gains and losses. In stark contrast to arm-level alterations, recurrent high-level focal amplifications and deletions were much less prevalent in these tumors. High-throughput mutation screening and exome sequencing revealed similarly low rates of somatic mutation in NETs (median of 0.77 non-silent mutations per megabase (Mb) of coding DNA) compared to other recent cancer exome sequencing efforts. Our analysis of this cohort identified only a single, statistically significant recurrent somatic mutation targeting the cyclin-dependent kinase inhibitor gene, CDKN1B, encoding p27.

Link

dbGap study = phs000579

Stichworte

  1. 27.10.22 27.10.22 - Simon Heim
  2. 13.12.22 13.12.22 - Kristina Keller
Rechteinhaber

Matthew Meyerson, MD, PhD, Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts, MA, USA

Hochgeladen am

13. Dezember 2022

DOI

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Lizenz

Creative Commons BY 4.0

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dbGaP phs000579 Small Intestine Neuroendocrine Tumors (Carcinoid Tumors)

Eligibility Criteria

Inclusion and exclusion criteria
Beschreibung

Inclusion and exclusion criteria

All samples with a pathological assessment of well-differentiated neuroendocrine tumors of the small intestine or metastasis of the small intestine and a signed informed consent were included as part of the study. Those samples with sufficient native genomic DNA were used for SNP arrays, whole-exome or whole-genome sequencing. Samples were removed from analysis when there was a poor concordance between tumor and normal SNPs. Samples that did not meet whole-exome capture and/or sequencing requirements were also removed from the analysis.
Beschreibung

All samples with a pathological assessment of well-differentiated neuroendocrine tumors of the small intestine or metastasis of the small intestine and a signed informed consent were included as part of the study. Those samples with sufficient native genomic DNA were used for SNP arrays, whole-exome or whole-genome sequencing. Samples were removed from analysis when there was a poor concordance between tumor and normal SNPs. Samples that did not meet whole-exome capture and/or sequencing requirements were also removed from the analysis.

Datentyp

boolean

Alias
UMLS CUI [1,1]
C1512693
UMLS CUI [1,2]
C0030664
UMLS CUI [1,3]
C0205615
UMLS CUI [1,4]
C0206754
UMLS CUI [1,5]
C0345832
UMLS CUI [1,6]
C0563486
UMLS CUI [1,7]
C0021430
UMLS CUI [1,8]
C3272453
UMLS CUI [1,9]
C3897601
UMLS CUI [1,10]
C3640076
UMLS CUI [1,11]
C3640077
UMLS CUI [2,1]
C0680251
UMLS CUI [2,2]
C0752046
UMLS CUI [2,3]
C5237802
UMLS CUI [2,4]
C1705241
UMLS CUI [3,1]
C0680251
UMLS CUI [3,2]
C4319199
UMLS CUI [3,3]
C5236167

Ähnliche Modelle

Eligibility Criteria

Name
Typ
Description | Question | Decode (Coded Value)
Datentyp
Alias
Item Group
Inclusion and exclusion criteria
All samples with a pathological assessment of well-differentiated neuroendocrine tumors of the small intestine or metastasis of the small intestine and a signed informed consent were included as part of the study. Those samples with sufficient native genomic DNA were used for SNP arrays, whole-exome or whole-genome sequencing. Samples were removed from analysis when there was a poor concordance between tumor and normal SNPs. Samples that did not meet whole-exome capture and/or sequencing requirements were also removed from the analysis.
Item
All samples with a pathological assessment of well-differentiated neuroendocrine tumors of the small intestine or metastasis of the small intestine and a signed informed consent were included as part of the study. Those samples with sufficient native genomic DNA were used for SNP arrays, whole-exome or whole-genome sequencing. Samples were removed from analysis when there was a poor concordance between tumor and normal SNPs. Samples that did not meet whole-exome capture and/or sequencing requirements were also removed from the analysis.
boolean
C1512693 (UMLS CUI [1,1])
C0030664 (UMLS CUI [1,2])
C0205615 (UMLS CUI [1,3])
C0206754 (UMLS CUI [1,4])
C0345832 (UMLS CUI [1,5])
C0563486 (UMLS CUI [1,6])
C0021430 (UMLS CUI [1,7])
C3272453 (UMLS CUI [1,8])
C3897601 (UMLS CUI [1,9])
C3640076 (UMLS CUI [1,10])
C3640077 (UMLS CUI [1,11])
C0680251 (UMLS CUI [2,1])
C0752046 (UMLS CUI [2,2])
C5237802 (UMLS CUI [2,3])
C1705241 (UMLS CUI [2,4])
C0680251 (UMLS CUI [3,1])
C4319199 (UMLS CUI [3,2])
C5236167 (UMLS CUI [3,3])

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