Metastatic CRPC samples were obtained from patients who died of CRPC in 2000-2013 and who signed written informed consent for a rapid autopsy performed within 6 h of death, under the aegis of the Prostate Cancer Donor Program at the University of Washington. Localized CRPC samples were obtained under written informed consent by transurethral resection of the prostate (TURP) procedures performed for obstructive uropathy at University of Texas Southwestern Medical Center. Hormone naive prostate cancer tissues from patients with biopsy-confirmed prostate cancer (Gleason Score 6-9) were obtained under written informed consent at the time of radical prostatectomy from the University of Texas Southwestern Medical Center tissue core under UTSW IRB STU 112013-056.