adults aged between 35 to 65 years old;

a minimum of 14 natural teeth, 10 of which should be posterior teeth;

periodontitis case definition was the presence of five teeth with a probing pocket depth of ≥ 5 mm and clinical attachment loss of ≥ 3 mm;

type 2 diabetes for at least 5 years and with glycated hemoglobin level > 7%.

smoking within the last 5 years;

pregnancy or lactating;

use of antibiotics or periodontal therapy in the previous six months;

concomitant medical therapy, except for diabetic condition;

other inflammatory conditions;

major diabetic complications such as retinopathy, nephropathy, neuropathy and atherosclerosis.